| 质粒名称: | pY018 (pcDNA3.1-hPdCpf1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69990 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5354 bp |
| 完整质粒大小: | 9400 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,CRISPR |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | hPdCpf1,Cpf1 |
| 物种来源: | Prevotella_disiens |
| 插入大小: | 4107 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | NLS和3xHA（C端子插入） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pY018 (pcDNA3.1-hPdCpf1) was a gift from Feng Zhang (Addgene plasmid # 69990 ; http://n2t.net/addgene:69990 ; RRID:Addgene_69990) |
| 参考文献部分: | Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
.png)
载体简介
载体序列
LOCUS pY018 (pcDNA3.1-hPdCpf1) 9515 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses humanized PdCpf1.
ACCESSION .
VERSION .
KEYWORDS pY018 (pcDNA3.1-hPdCpf1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9515)
AUTHORS Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS,
Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin
EV, Zhang F
TITLE Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas
System.
JOURNAL Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi:
10.1016/j.cell.2015.09.038.
PUBMED 26422227
REFERENCE 2 (bases 1 to 9515)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9515
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 16..395
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 396..599
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 550..570
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 644..663
/label=T7
/note="T7 promoter, forward primer"
promoter 644..662
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 704..713
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4679..4726
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 4733..4813
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
primer_bind complement(4887..4904)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4893..5117
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5163..5591
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5250..5269)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5460..5481
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5600..5620)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5605..5934
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5785..5920
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5847..5866
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6001..6795
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6055..6074)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6665..6684
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6969..7090
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7006..7025)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7060..7079
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7139..7155)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7139..7155)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7152..7174)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7163..7179
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7187..7217)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7232..7253
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7370..7387)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7541..8129)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7621..7640)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8300..9160)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8923..8942
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9161..9265)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9340..9359)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
601 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
661 gggagaccca agctggctag cgtttaaact taagcttggt accgccacca tggagaacta
721 tcaggagttc accaacctgt ttcagctgaa taagacactg agattcgagc tgaagcccat
781 cggcaagacc tgcgagctgc tggaggaggg caagatcttc gccagcggct cctttctgga
841 gaaggacaag gtgagggccg ataacgtgag ctacgtgaag aaggagatcg acaagaagca
901 caagatcttt atcgaggaga cactgagctc cttctctatc agcaacgatc tgctgaagca
961 gtactttgac tgctataatg agctgaaggc cttcaagaag gactgtaaga gcgatgagga
1021 ggaggtgaag aaaaccgccc tgcgcaacaa gtgtacctcc atccagaggg ccatgcgcga
1081 ggccatctct caggcctttc tgaagagccc ccagaagaag ctgctggcca tcaagaacct
1141 gatcgagaac gtgttcaagg ccgacgagaa tgtgcagcac ttctccgagt ttaccagcta
1201 tttctccggc tttgagacaa acagagagaa tttctactct gacgaggaga agtccacatc
1261 tatcgcctat aggctggtgc acgataacct gcctatcttc atcaagaaca tctacatctt
1321 cgagaagctg aaggagcagt tcgacgccaa gaccctgagc gagatcttcg agaactacaa
1381 gctgtatgtg gccggctcta gcctggatga ggtgttctcc ctggagtact ttaacaatac
1441 cctgacacag aagggcatcg acaactataa tgccgtgatc ggcaagatcg tgaaggagga
1501 taagcaggag atccagggcc tgaacgagca catcaacctg tataatcaga agcacaagga
1561 ccggagactg cccttcttta tctccctgaa gaagcagatc ctgtccgatc gggaggccct
1621 gtcttggctg cctgacatgt tcaagaatga ttctgaagtg atcaaggccc tgaagggctt
1681 ctacatcgag gacggctttg agaacaatgt gctgacacct ctggccaccc tgctgtcctc
1741 tctggataag tacaacctga atggcatctt tatccgcaac aatgaggccc tgagctccct
1801 gtcccagaac gtgtatcgga atttttctat cgacgaggcc atcgatgcca acgccgagct
1861 gcagaccttc aacaattacg agctgatcgc caatgccctg cgcgccaaga tcaagaagga
1921 gacaaagcag ggccggaagt ctttcgagaa gtacgaggag tatatcgata agaaggtgaa
1981 ggccatcgac agcctgtcca tccaggagat caacgagctg gtggagaatt acgtgagcga
2041 gtttaactct aatagcggca acatgccaag aaaggtggag gactacttca gcctgatgag
2101 gaagggcgac ttcggctcca acgatctgat cgaaaatatc aagaccaagc tgagcgccgc
2161 agagaagctg ctgggcacaa agtaccagga gacagccaag gacatcttca agaaggatga
2221 gaactccaag ctgatcaagg agctgctgga cgccaccaag cagttccagc actttatcaa
2281 gccactgctg ggcacaggcg aggaggcaga tcgggacctg gtgttctacg gcgattttct
2341 gcccctgtat gagaagtttg aggagctgac cctgctgtat aacaaggtgc ggaatagact
2401 gacacagaag ccctattcca aggacaagat ccgcctgtgc ttcaacaagc ctaagctgat
2461 gacaggctgg gtggattcca agaccgagaa gtctgacaac ggcacacagt acggcggcta
2521 tctgtttcgg aagaagaatg agatcggcga gtacgattat tttctgggca tctctagcaa
2581 ggcccagctg ttcagaaaga acgaggccgt gatcggcgac tacgagaggc tggattacta
2641 tcagccaaag gccaatacca tctacggctc tgcctatgag ggcgagaaca gctacaagga
2701 ggacaagaag cggctgaaca aagtgatcat cgcctatatc gagcagatca agcagacaaa
2761 catcaagaag tctatcatcg agtccatctc taagtatcct aatatcagcg acgatgacaa
2821 ggtgacccca tcctctctgc tggagaagat caagaaggtg tctatcgaca gctacaacgg
2881 catcctgtcc ttcaagtctt ttcagagcgt gaacaaggaa gtgatcgata acctgctgaa
2941 aaccatcagc cccctgaaga acaaggccga gtttctggac ctgatcaata aggattatca
3001 gatcttcacc gaggtgcagg ccgtgatcga cgagatctgc aagcagaaaa ccttcatcta
3061 ctttccaatc tccaacgtgg agctggagaa ggagatgggc gataaggaca agcccctgtg
3121 cctgttccag atcagcaata aggatctgtc cttcgccaag acctttagcg ccaacctgcg
3181 gaagaagaga ggcgccgaga atctgcacac aatgctgttt aaggccctga tggagggcaa
3241 ccaggataat ctggacctgg gctctggcgc catcttctac agagccaaga gcctggacgg
3301 caacaagccc acacaccctg ccaatgaggc catcaagtgt aggaacgtgg ccaataagga
3361 taaggtgtcc ctgttcacct acgacatcta taagaacagg cgctacatgg agaataagtt
3421 cctgtttcac ctgagcatcg tgcagaacta taaggccgcc aatgactccg cccagctgaa
3481 cagctccgcc accgagtata tcagaaaggc cgatgacctg cacatcatcg gcatcgatag
3541 gggcgagcgc aatctgctgt actattccgt gatcgatatg aagggcaaca tcgtggagca
3601 ggactctctg aatatcatca ggaacaatga cctggagaca gattaccacg acctgctgga
3661 taagagggag aaggagcgca aggccaaccg gcagaattgg gaggccgtgg agggcatcaa
3721 ggacctgaag aagggctacc tgagccaggc cgtgcaccag atcgcccagc tgatgctgaa
3781 gtataacgcc atcatcgccc tggaggatct gggccagatg tttgtgaccc gcggccagaa
3841 gatcgagaag gccgtgtacc agcagttcga gaagagcctg gtggataagc tgtcctacct
3901 ggtggacaag aagcggcctt ataatgagct gggcggcatc ctgaaggcct accagctggc
3961 ctctagcatc accaagaaca attctgacaa gcagaacggc ttcctgtttt atgtgccagc
4021 ctggaataca agcaagatcg atcccgtgac cggctttaca gacctgctgc ggcccaaggc
4081 catgaccatc aaggaggccc aggacttctt tggcgccttc gataacatct cttacaatga
4141 caagggctat ttcgagtttg agacaaacta cgacaagttt aagatcagaa tgaagagcgc
4201 ccagaccagg tggacaatct gcaccttcgg caatcggatc aagagaaaga aggataagaa
4261 ctactggaat tatgaggagg tggagctgac cgaggagttc aagaagctgt ttaaggacag
4321 caacatcgat tacgagaact gtaatctgaa ggaggagatc cagaacaagg acaatcgcaa
4381 gttctttgat gacctgatca agctgctgca gctgacactg cagatgcgga actccgatga
4441 caagggcaat gattatatca tctctcctgt ggccaacgcc gagggccagt tctttgactc
4501 ccgcaatggc gataagaagc tgccactgga tgcagacgca aacggagcct acaatatcgc
4561 ccgcaagggc ctgtggaaca tccggcagat caagcagacc aagaacgaca agaagctgaa
4621 tctgagcatc tcctctacag agtggctgga tttcgtgcgg gagaagcctt acctgaagaa
4681 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat cctacccata
4741 cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc catatgatgt
4801 ccccgactat gcctaagaat tctgcagata tccagcacag tggcggccgc tcgagtctag
4861 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
4921 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
4981 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
5041 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
5101 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
5161 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
5581 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
5641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
5701 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
5761 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
5821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
5881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
5941 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
6001 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
6061 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
6121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
6181 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
6241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
6301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
6361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
6421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
6481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
6541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
6601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
6661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
6721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
6781 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
6841 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
6901 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
6961 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
7021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
7081 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
7141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
7201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
7261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
7321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
7381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
7441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
7501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
7561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
7621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
7681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
7741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
7801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
7861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
7921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
7981 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
8041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
8101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
8161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
8221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
8281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
8341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
8401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
8461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
8521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
8581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
8641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
8701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
8761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
8821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
8881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
8941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
9001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
9061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
9121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
9181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
9241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
9301 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
9361 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
9421 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
9481 ggttaggcgt tttgcgctgc ttcgcgatgt acggg
//
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